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KMID : 0613820100200101549
Journal of Life Science
2010 Volume.20 No. 10 p.1549 ~ p.1555
Antimicrobial Susceptibility and Biochemical Characteristics of Streptococcus suis Isolated from Diseased Pigs in Gyeongbuk Province
Choi Seong-kyoon

Kim Seong-Guk
Kim Young-Hoan
Choi Jung-Hye
Cho Min-Hee
Cho Gil-Jae
Abstract
Streptococcus suis is a worldwide pathogen of a variety of porcine infection and has also been described as a pathogen for humans. We studied biochemical characteristics, antimicrobial susceptibility, and identification of polymerase chain reaction (PCR) of S. suis isolated from diseased pigs in Gyeongbuk province from 2004 to 2009. Sixty-one isolates were identified as S. suis by biochemical characteristics and PCR from 40 farms. The biochemical characteristics of S. suis isolates were production of VP-negative, hippurate, esculin, and arginine decarboxylase-positive, and fermentation of carbohydrate was variable lactose, trehalose, inulin, and raffinose, which was typeable 11 phenotype. In an antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone and florfenicol, while being highly resistant to streptomycin, kanamycin, amikacin, neomycin, erythromycin, clindamycin, and tetracycline. The isolates were divided into 11 phenotypes of biochemistry. By using PCR, the 16S-rRNA gene DNA fragment was detected at 304 bp frm all of isolates. These results may provide the basic information needed to establish strategies for the prevention of S. suis infection in pigs.
KEYWORD
Streptococcus suis., antimicrobial susceptibility, biochemical characteristics, PCR
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